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What Enzyme forms Covalent Bonds between Restriction fragments?

  1. DNA primase
  2. DNA helicase
  3. single-stranded binding protein
  4. DNA polymerase
  5. DNA ligase

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5. DNA ligase

DNA ligase is the enzyme that forms Covalent Bonds between Restriction fragments.


The enzyme DNA ligase joins the two DNA molecules by covalent bonds. This enzyme, which the cell normally uses in DNA replication is a DNA pasting enzyme that catalyzes the formation of covalent bonds between adjacent nucleotides, joining the strands.

The result is a recombinant DNA plasmid.

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